Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Proteomics Qualitative Analysis in Plant
INQUIRYIntroductions
In-depth and systematic research on proteins as the functional executives of life activities can not only reveal the essence of life activities in a panoramic manner, but also the key proteins discovered are of great significance for revealing the occurrence and development mechanisms of plant growth and resistance to adversity and stress. Qualitative protein analysis is mainly to determine whether there is protein in the sample and what kind of protein is present. It mainly uses liquid mass spectrometry to detect the peptides after enzymatic hydrolysis, and then compares with the protein sequence library of the corresponding species to obtain which proteins are present in the sample, as well as the peptide sequences and isoelectric points of these proteins, etc.
Services
Service content
- Sample processing in the form of protein samples / gel strips / IP protein products, such as proteolysis, desalination, etc.
- Mass detection
- Database search
- Search results and data report
Workflow of proteomics qualitative analysis
Analysis content
- Raw data of mass spectrometry
- Protein library search
- Data quality control
- Protein identification
- Protein function annotation
GO function notes
KEGG pathway notes
COG function notes
Sample requirements
- Co-IP / Pull down / SDS-Page strips and other protein solutions
- Protein solution > 20 μl, concentration > 100 μg / μl
- Protein freeze-dried powder > 100 μg
- The strips of the adhesive strips (test-stained, silver-stained-MS compatible) are clearly visible to the naked eye.
Project cycle
- It takes 15~30 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental procedures, related mass spectrometry parameters, partial mass spectrometry pictures, and data retrieval report.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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