Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Whole Genome Resequencing Service
INQUIRYINTRODUCTIONS
Plant genome resequencing is a sequencing performed when the genome of a plant species is known. By re-sequencing the genome of different individuals of the plant species or different tissues of the same individual, the differences in genome sequence can be fully discovered at the population or individual level. This method can obtain a large amount of accurate genomic information at a low cost, and has great guiding significance for plant Breeding research. This technology has been widely used in various fields, such as plant individual genome variation detection, plant mutant functional mutation site location, plant population genetic analysis, plant whole gene association analysis, etc.
SERVICES
With years of rich experience, Lifeasible provides plant whole genome resequencing service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of plant whole genome resequencing service
Analysis content
| Data quality control | Remove joint contamination and low-quality data Compare with the reference sequence and count the sequencing depth and coverage |
| Standard analysis | Basic analysis of original data Sequence comparison SNP/ InDel/ SVI CNV detection Annotation and statistics Genome variation Circos map display, etc. |
| Advanced analysis |
Screening based on the harmfulness of variants 1. Screening of mutation sites 2. Mutation Site Harmfulness Classification (ACMG) 3. Non-coding region mutation site screening 4. Structural variation CNV/SV harmfulness analysis |
| Screening based on selection information 1. Dominantrecessive inheritance pattern analysis (must provide a pedigree map) 2. Family linkage analysis (family sample) or new mutation screening (core family) 3. Homozygous area (ROH) analysis 4. Shared mutation gene screening (distribute samples) | |
| Screening based on gene function and phenotype 1. Candidate gene function enrichment analysis 2. Candidate gene pathway enrichment analysis 3. Candidate genes and disease relevance ranking |
Sample requirements
- Sample concentration≥30ng/μl, total amount≥6μg, OD260/280＝1.8~2.0.
(The amount of DNA required is related to the number of libraries constructed and the type of library)
Project cycle
- The entire project generally takes about 2 months, and the specific completion time depends on the size and complexity of the genome. The time is calculated from the time the sample is qualified, and does not include the time when the experiment is stalled due to sample problems.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- Our experienced researchers help you with scientific plan design.
- Our method is excellent, the cycle is short, and the accuracy is high.
- Excellent analysis creates project results.
- We combine rich project experience, professional project planning guidance and analysis process to ensure rapid and accurate assessment of genome characteristics.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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