Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
CHIP-seq in Plant
INQUIRYIntroductions
Chromatin immunoprecipitation sequencing (ChIP-seq) is a technology used to study protein-specific binding DNA fragments, histone modifications and epigenetic modifications in the whole genome. Studying these three themes helps to gain insight into the regulation of protein on gene expression and the functional structure of chromosomes. It has been widely used in the research of human, mouse, yeast, rice, Arabidopsis and other species. The main applications in the field of agricultural molecular breeding are as follows: 1) Judge the type of histone modification at a specific position in the DNA chain. 2) Precisely locate the binding sites of RNA polymerase II and other trans factors on the genome. 3) Study the relationship between histone covalent modification and gene expression. 4) Study the binding sites and functions of transcription factors, etc.
Services
With years of rich experience, Lifeasible provides CHIP sequencing and analysis services. Customers only need to provide tissues or DNA sample, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of CHIP-seq in plant
Analysis content
| Analysis content | Solved problem |
|---|---|
| Sequencing data quality assessment | Filter out low-quality data to ensure data quality |
| Align with reference genome | SCC analysis to determine the effect of IP |
| Peak calling | Analyze protein binding sites |
| Motif analysis | Protein binding sequence preference |
| Peak related gene annotation | Looking for potential regulatory genes for proteins |
| Difference peak analysis | Analyze the difference between different samples peak |
| Function analysis of related gene | GO / KEGG enrichment analysis of related gene |
Sample requirements
- DNA ＞ 250 ng
It is necessary to provide a gel image for detecting DNA breaks. The main band of the DNA electrophoresis after the break is required to be in the range of 100~500 bp, and it is recommended to be around 250 bp.
Project cycle
- For cell line samples, it takes about 90 working days to complete the standard process.
- For DNA samples after immunoprecipitation, it takes about 45 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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