Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Genome Editing with TALEN
INQUIRYIntroductions
TALEN (transcription activator-like effector nuclease) is an artificially modified restriction endonuclease, composed of a DNA recognition domain and an endonuclease cleavage domain (Fok I). The principle of TALEN technology is not complicated. First, the DNA recognition element of TALEN binds to the target DNA site. Secondly, under the action of FokI nuclease, the specific site is cut. Finally, with the help of the inherent homology-directed repair (HDR) or non-homologous end joining pathway (NHEJ) in the cell, specific sequences are repaired, that is gene knockout, gene knock-in, and specific site mutation. Today, TALEN technology has been widely used in corn, rice, wheat, barley and other crops. The targeted modification of endogenous genes in crops by TALEN gene editing technology provides a new way for crop genetic improvement. Using this technology to delete large chromosomal segments can quickly knock out gene clusters that control undesirable traits, and achieve rapid genetic improvement of crops.
Services
Lifeasible provides TALEN services to global customers. With an experienced team, advanced technology and state-of-the-art facilities, we can provide complete customized services for TALEN-based targeted genome modification research, including TALEN design and construction, functional verification and production of genetically modified plants. At Lifeasible, we will analyze each project and propose the best plan to ensure that various needs are met, thus accelerating your research plan.
Service content
| TALEN | TALE-TF |
|---|---|
| Genetic modification | Gene activation |
| Gene knockout | Gene repression |
| Gene knock-in | Others |
| Others |
※ The service content is constantly updated, please contact our staff by email for more up-to-date and relevant information
Service process
- Experimental design
- Functional verification service
- Transgenic plant service
- Deliver
Customer provided
- Species
- Gene name or gene ID
Delivery standard
- Genetically modified plants
- Images
- Complete project report (materials, reagents, instruments, methods, data analysis, experimental results)
Project cycle
- Takes about 2 months
Advantages
- No restriction on gene sequence, cell or species.
- The success rate is almost 100%. The off-target rate is low, and there is no obvious off-target effect yet.
- The target is highly sequence specific on the genome.
- It overcomes the problems that the conventional ZFN method cannot recognize any target gene sequence, and the recognition sequence is often affected by upstream and downstream sequences, and has higher targeting activity than ZFN.
- TALEN technology is a gene knockout at the DNA level, completely solving the bottleneck of RNA interference technology knocking down only at the mRNA level.
- The experiment design is simple and accurate, the experiment period is short, and the cost is low.
- It Can be applied to gene knockout, gene knock-in, gene modification, gene activation or inhibition, etc.
- Flexible binding domain and functional domain design, you can choose TALEN, TALE-TF or other TAL effectors.
※ It should be noted that our service is only used for research.
Online Inquiry
