Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
INQUIRYIntroductions
Targeted proteomics is a science developed on the basis of proteomics for quantitative research of specific target proteins. Compared with non-targeted proteomics, targeted proteomics selectively selects ions for mass spectrometry data collection, so it can be used for the analysis of a large number of samples and has better accuracy, sensitivity and reproducibility. Targeted proteomics can be applied to system-level studies of post-translational modification, protein conformation, protein-protein interaction, kinetics, metabolic and signal transduction pathways. Targeted proteomics quantitative technology mainly includes MRM (multiple reaction monitoring) and PRM (parallel reaction monitoring). MRM, also known as SRM (selected reaction monitoring), uses triple quadrupole mass spectrometry (QQQ-MS) to collect signals that conform to the target ion rule, so as to achieve relative or absolute quantification of the target protein. PRM is derived from MRM, combining the high selectivity of the quadrupole and the high resolution and high precision of Orbitrap. After fragmenting the selected precursor ion, it will measure all fragment ions, which has better detection sensitivity and anti-interference ability.
Services
The absolute protein quantification strategy is an effective detection method for protein quantification and post-translational modification. Lifeasible uses a high-throughput mass spectrometry platform to provide targeted proteomics analysis services, including MRM and PRM quantitative proteomics analysis, as well as absolute quantitative analysis.
Protein targeted quantitative service
- PRM - Parallel Reaction Monitorin
- MRM - Multiple Reaction Monitoring
- AQUA - Automated Quantitative Analysis System
Workflow of protein targeted quantitative service (PRM / MRM)
Analysis content (MRM)
- MRM spectrum analysis, protein data quality evaluation
- PCA analysis of multi-component samples
- Identified protein functional annotations (GO/KEGG functional annotations)
- Statistical analysis of differentially expressed proteins
- Cluster analysis of differentially expressed proteins
- Differential protein interaction analysis
Venn diagram, Volcano Plot
Hierarchical clustering, K-means clustering
Protein interaction network analysis based on STRING database
Sample requirements
- Fresh plant tissue > 1 g
- The total amount of protein in the protein solution > 100 μg
Project cycle
- It takes 50~70 working days to complete the standard process.
Feedback to customers
- Raw data
- A project report includes experimental procedures, related mass spectrometry parameters, and standard analysis results.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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