Plant Genome Editing with CRISPR / Cas9

INQUIRY

Introductions

CRISPR / Cas9 genome editing technology has become a revolutionary tool in the field of life sciences, providing efficient, fast and cheap genetic manipulation tools for basic research in botany and genetic improvement of crops. The CRISPR / Cas9 system can achieve precise knock-out and knock-in genetic operations, and can also be used to target activation or inhibition of gene expression. Crop varieties improved using CRISPR / Cas9 technology have also emerged, which will surely promote the application and development of precision genome editing technology in crop genetic improvement. Today, CRISPR technology has been used in the transformation of many plant genomes, including model organisms such as Arabidopsis thaliana and Medicago truncatula, and important economic crops such as potatoes, corn, wheat, rice and mushrooms.

Schematic of Cas9 / gRNA genome editing (Yuduan D, et al., 2016)

Services

The plant genome editing technology via CRISPR / Cas9 has been widely used in agricultural molecular breeding, such as gene function research, crop breeding and improvement research, mutation library construction, transcription regulation research, epigenetic modification research, etc. Lifeasible provide one-stop services of plant genome editing with CRISPR / Cas9, including gene knockout, gene knock-in and gene deletion and others. With an excellent platform, our experienced scientists are committed to providing reliable and satisfactory services to our customers.

Service content of typical species

Service Items	Typical Species	Services Content
Genome editing in model plants	Arabidopsis thaliana Nicotiana tabacum	Single gene knockout Multiplex genome editing
Genome editing in crops	Oryza sativa Triticum aestivum Solanum lycopersicum	sgRNA design and construction Expression vector construction Plant genetic transformation Identification of mutants Off-target effects analysis T1 seeds harvesting
Genome editing without genetic modification in plants	Oryza sativa Arabidopsis thaliana Solanum lycopersicum	Selection of targets Construction of CRISPR vector or preparation of RNP Plant transformation and regeneration with / without selection Mutation identification in T0 plants Screening of transgene-free edited plants in T1 generation

Basic services of CRISPR

• SgRNA design and validation
• Basic editing of CRISPR

Gene knockout

Gene (fragment) site-directed insertion

Gene (fragment) site-directed substitution

Single base editing

Gene expression regulation

• Custom CRISPR/Cas9 HDR vector
• Single-stranded DNA synthesis
• CRISPR off-target analysis
• Custom CRISPRa / CRISPRi sgRNA vector
• sgRNA Bioinformatics

※ The project is constantly updated, please contact our staff by email for more up-to-date and relevant information.

Service process

• Design and construction of sgRNA
• Transform Cas9 and sgRNA into plant cells
• Targeted mutation analysis
• Delivery

Project cycle

• Takes about 2 months

Advantages

• High efficiency
• Traceless genome editing
• Standardization system, scientific management
• Leading technology platform conditions, first-class R&D and production equipment

Reference

1. Yuduan D, Hong L, Ling-Ling C, et al. Recent Advances in Genome Editing Using CRISPR/Cas9[J]. Frontiers in Plant Science, 2016, 7.

 

※ For research or industrial use.

Related Sections

• Plant Genome Editing with TALEN
• Plant Genome Editing with ZFN
• RNAi Mediated Plant Gene Silencing
• Overexpression of Plant Genes
• Virus-Induced Gene Silencing (VIGS) of Plant Genes

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