Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Genome-Wide Association Study (GWAS) of Plant
INQUIRYINTRODUCTIONS
Genome-wide association study (GWAS) is a research strategy for studying the phenotype-genotype relationship at the population level. GWAS is to re-sequence the whole genome of each individual in a population with rich genetic diversity, and detect the genotype information of millions of molecular markers (SNP, CNV, etc.). Then combined with the phenotype data of the target trait, based on certain statistical methods, the correlation analysis between the individual phenotype and genotype in the population is carried out to quickly obtain the chromosome segment or gene locus that affects the phenotypic variation of the target trait, so as to analyze the complex impact Genetic variation of traits. This technology is mainly used in the research of various quantitative traits of animals and plants and the research of various human phenotypes/complex diseases.
SERVICES
With years of rich experience, Lifeasible provides genome-wide association study (GWAS) service. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of genome-wide association study (GWAS)
Analysis content
- Standard analysis
Raw data filtering
Data comparison
SNP and indel detection
- Advanced analysis
Linkage disequilibrium analysis
Analysis of population structure (PCA, evolutionary tree construction, group structure analysis)
Polymorphism analysis
Candidate gene extraction and mutation annotation
- Personality analysis
Phenotypic analysis
Genotype analysis
GWAS analysis (simple generalized linear model analysis, generalized linear model analysis, mixed linear model analysis, K+Q linear model analysis)
Sample requirements
- Whole genome resequencing
Sample concentration≥30ng/μl, total amount≥6μg, OD260/280＝1.8~2.0.
- Reduced-representation genome sequencing
Sample concentration≥25 ng/μl, total amount≥2μg, OD260/280＝1.8~2.0
- Recommended sample size is greater than 300
(The amount of DNA required is related to the number of libraries constructed and the type of library)
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
ADVANTAGES
- Whole-genome association analysis uses the advanced Illumina sequencing platform to quickly and efficiently read high-quality sequencing data.
- Lifeasible has a high-performance computing platform (HPC) that uses an efficient combination of DELL computing nodes and Isilon storage to achieve fast and stable sequencing data analysis and delivery.
- Our experienced researchers help you with scientific plan design.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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