Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant In Situ Hybridization Service
INQUIRYIntroductions
As a research tool of molecular biology, in situ hybridization is a novel and prominent technique in plant genome analysis. It is a key advance in the identification and characterization of hybrid and offspring genome development through classical breeding methods. In situ hybridization is a technology for accurately quantifying and locating specific nucleic acid sequences. Through this technology, we can analyze genetic organization, genome composition and genome reorganization, and can also detect cRNA, miRNA, LnRNA, DNA, specimens of various species, and even tissues Microarray. The principle of in situ hybridization is to use a labeled single-stranded nucleic acid containing a specific sequence as a probe to hybridize with a single-stranded nucleic acid (target nucleic acid) in a tissue or cell under appropriate conditions. Autoradiography or immunocytochemistry is then used to detect the labeled probes to display specific DNA or RNA molecules in situ in the tissue or cell.
Services
Lifeasible is a global company specializing in agricultural molecular breeding. With years of experience in agricultural biotechnology, we provide a series of services, including research on nucleic acids, proteins, cells, tissues and other different levels. At the nucleic acid level, such as the cloning, identification, expression, functional analysis of plant genes and the production of plant products, to help researchers understand the biological functions of plant genes and master these functions to regulate plant growth, stress resistance, disease prevention and treatment, and the cultivation and selection of required plant traits. As part of its trait introgression services, Lifeasible has established an in situ hybridization platform to meet your related needs and serve you wholeheartedly.
Workflow of in situ hybridization
Provided by customer
- Sample information to be tested
- Sample size information and testing requirements, etc.
- The target gene sequence to be tested, gene ID or accession number, etc.
Provided by Lifeasible
- Experimental materials such as remaining probes
- Section after hybridization
- Original picture
- Complete project report (materials, reagents, instruments, methods, data analysis, experimental results)
Advantages
- Reagents and probes are non-radioactive, safe and controllable
- The probe is stable and can be stored for a long time
- The experiment has high specificity and accuracy
- Repeatability of experimental results to ensure the accuracy of results.
- Quality after-sales service.
- There is no need to extract nucleic acid from tissue cells, it has high sensitivity to target sequences with extremely low content in tissues, and can maintain the integrity of tissue and cell structure, reflecting the location of specific nucleic acid molecules.
※ It should be noted that our service is only used for research.
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