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Plant In Situ Hybridization Service

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Introductions

In situ hybridization

As a research tool of molecular biology, in situ hybridization is a novel and prominent technique in plant genome analysis. It is a key advance in the identification and characterization of hybrid and offspring genome development through classical breeding methods. In situ hybridization is a technology for accurately quantifying and locating specific nucleic acid sequences. Through this technology, we can analyze genetic organization, genome composition and genome reorganization, and can also detect cRNA, miRNA, LnRNA, DNA, specimens of various species, and even tissues Microarray. The principle of in situ hybridization is to use a labeled single-stranded nucleic acid containing a specific sequence as a probe to hybridize with a single-stranded nucleic acid (target nucleic acid) in a tissue or cell under appropriate conditions. Autoradiography or immunocytochemistry is then used to detect the labeled probes to display specific DNA or RNA molecules in situ in the tissue or cell.

Services

Lifeasible is a global company specializing in agricultural molecular breeding. With years of experience in agricultural biotechnology, we provide a series of services, including research on nucleic acids, proteins, cells, tissues and other different levels. At the nucleic acid level, such as the cloning, identification, expression, functional analysis of plant genes and the production of plant products, to help researchers understand the biological functions of plant genes and master these functions to regulate plant growth, stress resistance, disease prevention and treatment, and the cultivation and selection of required plant traits. As part of its trait introgression services, Lifeasible has established an in situ hybridization platform to meet your related needs and serve you wholeheartedly.  

Workflow of in situ hybridization

Workflow of In Situ Hybridization - Lifeasible

Provided by customer

  • Sample information to be tested
  • Sample size information and testing requirements, etc.
  • The target gene sequence to be tested, gene ID or accession number, etc.

Provided by Lifeasible

  • Experimental materials such as remaining probes
  • Section after hybridization
  • Original picture
  • Complete project report (materials, reagents, instruments, methods, data analysis, experimental results)

Advantages

  • Reagents and probes are non-radioactive, safe and controllable
  • The probe is stable and can be stored for a long time
  • The experiment has high specificity and accuracy
  • Repeatability of experimental results to ensure the accuracy of results.
  • Quality after-sales service.
  • There is no need to extract nucleic acid from tissue cells, it has high sensitivity to target sequences with extremely low content in tissues, and can maintain the integrity of tissue and cell structure, reflecting the location of specific nucleic acid molecules.

※ For research or industrial use.

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