Wheat Exome Capture Sequencing Service

INQUIRY

As one of the world's most important food crops, the yield and quality of wheat directly impact global food security. The common wheat genome is large and characterized by a hexaploid structure, with a high proportion of repetitive sequences. Traditional whole-genome sequencing faces challenges, such as massive data volume, complex analysis, and high cost. Wheat whole-exome capture sequencing employs high-throughput sequencing technology to target exome sequencing. This approach enables direct identification of genetic mutations associated with protein functional variations and facilitates research on coding sequences directly related to gene function, significantly reducing sequencing costs. Leveraging an advanced plant whole-exome capture sequencing platform, Lifeasible offers wheat whole-exome capture sequencing services. By precisely focusing on exonic regions, we empower the advancement of wheat research and breeding programs.

The Significance of Wheat Exome Capture Sequencing

• Focus on functional variants. Exon capture sequencing directly targets protein-coding regions, by passing redundant sequencing of extensive non-coding areas. This approach significantly reduces data volume while enhancing the accuracy and efficiency of functional variant discovery.
• Supporting wheat molecular breeding and trait improvement. By profiling exonic variations across different varieties or mutants, functional genes associated with target traits can be rapidly identified, providing target references for marker-assisted selection (MAS) and gene editing.
• Supporting population genetics and evolutionary studies. Exome capture sequencing enables variation resolution across wheat subgenomes, advancing research into domestication, subspecies differentiation, and gene family evolution.
• Reducing costs while balancing depth and coverage. Compared to whole-genome resequencing, exome capture sequencing achieves sufficient coverage depth with substantially less sequencing data, offering an economically viable solution for large-scale sample studies.

Our Service for Wheat Exome Capture Sequencing

Our wheat whole-exome capture sequencing service  provides efficient and comprehensive coverage of protein-coding regions within the wheat genome, enabling researchers to gain a comprehensive understanding of its structure and function. This facilitates the elucidation of wheat genetic traits, identification of key genes, and in-depth investigation of related physiological processes. Futhermore, The technical service also enables researchers to rapidly and accurately identify mutation sites within the wheat genome. By sequencing and analyzing large sample populations, key mutation sites associated with important agronomic traits such as yield and disease resistance can be identified. This information serves as a foundation for directed improvement efforts, enabling precision breeding.

Applications of Wheat Exome Capture Sequencing

• Molecular breeding. Rapidly screen genes associated with stress tolerance, high yield, and superior quality traits to accelerate the development of elite wheat varieties.
• Population genetics. Analyze genetic diversity through large-scale population sequencing to identify key loci for domestication and adaptive evolution.
• Functional genomics. Precisely map gene functions to advance wheat genetic improvement.

Our Service Process

• Sample receipt and testing. Conduct quality control testing on DNA samples.
• Library construction and exon capture. Perform DNA fragmentation, adapter ligation, PCR amplification, and exon region hybridization capture.
• High-throughput sequencing. Perform high-throughput sequencing on target samples.
• Data quality control and analysis. This process includes data filtering, alignment, variant detection, annotation, and statistical analysis.
• Reporting and delivery. Provision of raw sequencing data, variant site tables, functional annotation results, and graphical reports.

Fig.2 Our service process. (Lifeasible)

Highlights of Our Wheat Exome Capture Sequencing

• Flexible customization and end-to-end services. We support adding SNP, Indel, or structural variant sites based on client requirements, enhancing exon region design for specific varieties.
• One-stop service. We provide a comprehensive solution from sample extraction, library construction, hybridization capture, to data analysis, compatible with automated equipment, to significantly shorten the experimental cycle.
• Extensive experience. We possess deep expertise in wheat and other polyploid plant genomics, enabling precise alignment and variant analysis tailored to polyploid structures.
• High-efficiency exon capture system. Our optimized probe design and hybridization conditions enhance capture uniformity and target coverage, ensuring robust data quality and downstream analysis reliability.

Whole-exome capture sequencing technology for wheat offers an efficient, cost-effective, and precise solution for identifying functional variations within complex genomes. Leveraging advanced capture and sequencing platforms, a specialized data analysis team, and a comprehensive service system, Lifeasible is committed to providing reliable genomic-level support for global wheat research and breeding. If you are interested, please feel free to contact us.

 

※ For research or industrial use.

Related Sections

• Plant Genome De Novo Service
• Plant Whole Genome Resequencing Service
• Plant Reduced-Representation Genome Sequencing (RRGS)
• Plant Genetic Map Service
• BSA Trait Positioning of Plant
• Genome-Wide Association Study (GWAS) of Plant
• eQTL Analysis of Plant
• Plant Genetic Evolution Service
• Plant Pan-genome Sequencing
• Plant Whole Exome Sequencing Service
• Individual Selection Pressure Analysis of Plant
• Mixing-tank Selection Pressure Analysis of Plant
• Plant Whole Genome Survey
• Plant Exon Capture Services

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