Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant STR / SSR Analysis Service
INQUIRYIntroductions
Short tandem repeats (STR), also known as simple sepeats (SSR) or microsatellite, are widely distributed in the genome of eukaryotes. Generally, it is composed of a core sequence of 2 to 6 bp which is repeatedly arranged in series, and the number of repetitions is mostly between 10 and 60. There is a high degree of variation in the number of repetitive units in SSR, which manifests as euploid variation in the number of microsatellites or the sequence of the repetitive unit sequence may not be exactly the same, resulting in polymorphisms at multiple sites. The polymorphism of different SSRs among different species and even different individuals. SSR markers can reveal these variations well. Because SSR has the advantages of wide and uniform distribution, rich polymorphism, large amount of information, and simple detection, it has been widely used in many fields such as genetic mapping, kinship identification, linkage analysis, gene mapping, genetic breeding and so on.
Services
With years of rich experience, Lifeasible provides STR / SSR analysis service in plant. We provide a one-stop service model. As long as you provide experimental samples, we will complete a series of processes from sample extraction to data analysis.
Analytical method
We use the ABI genetic analyzer to detect the fluorescently labeled DNA fragments, combined with the molecular weight internal standard to calculate the length of the DNA fragments, so that STR typing becomes more efficient and faster, and the results are more accurate.
Experimental workflow of STR / SSR analysis
Sample requirements
- DNA ≥ 20 μl, concentration ≥ 50 ng / μl
- Fresh tissue ≥ 300 mg
- It is necessary to provide the information of the STR site and the exact sequence of 200bp upstream and downstream in the STR site.
Project cycle
- A standard service process takes about 4~8 weeks, please consult our technicians for details.
Feedback to customers
- STR typing results (EXCEL) and graph generated by GeneMapper (PDF)
- The primer sequence designed for the amplification reaction system
- Other information required by the customer
Service process
- Submit the relevant requirements and materials of the project.
- Discuss project details with our professional technical team.
- Revise the experimental plan based on the opinions after discussion.
- Both parties are satisfied and reach an agreement to sign a technical service contract.
- Carry out experiments and complete the content stipulated in the contract on time.
- After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- Advanced equipment, mature and stable methods.
- Optimized experimental procedures ensure extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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