Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Oryza sativa Transformation
INQUIRYIntroductions
Rice is an important cereal crop of annual gramineous family, herbaceous rice genus, and monocot. Its Latin name is Oryza sativa. It likes high temperature, high humidity and short daylight. Rice has the following characteristics, so it is used as a model plant in monocots.
- Self-pollinating, 24 chromosomes, diploid plant
- The genome sequence is known, with a smaller genome
- Easy to cultivate and grow faster
- A mature Agrobacterium-mediated rice genetic transformation system has been established
- High collinearity with other cereals
As a model plant, rice is often used for research on gene function and regulation, aiming to improve agronomic traits, such as enhancing resistance to biotic or abiotic stress.
Services
Lifeasible has established a rice transformation platform through Agrobacterium-mediated method. The platform has a complete, large-scale, standardized technical system, and provides professional and convenient technologies for rice genetic transformation for agricultural personnel who serve rice as the research object.
Customer provided materials
- The sequence of the target gene and the Gene Bank ID
- Plasmids, Agrobacterium, etc.
- Vector to be transformed (conventional vector can also be provided by us)
- Unconventional receptor material
※ If the gene to be modified is a gene related to abortion, lethality, or development, or flowering, we cannot guarantee the results and cycle of the experiment.
Receptor material
- Recipient material varieties:
- Recipient material types: rice callus
Japonica varieties: Zhonghua 11, Nipponbare, Nipponbare, Akdong, Suweon330, Anjungbyeo, Taipei309, Cheniere and Katy, etc.
Indica varieties: Indica rice 9311, Minghui86, Minghui63, Kasalath, ASD16, White Ponni, BR29 and IR64, etc.
Transformation methods
- Co-cultivation of Agrobacterium and callus obtained from mature seeds.
- Co-cultivation of Agrobacterium and immature embryos.
- Co-cultivation of rice callus and Agrobacterium on filter paper infiltrated with liquid medium
- Co-cultivation of Agrobacterium and suspension cells.
- The apical embryonic tissues of the mature seeds after soaking are punctured with a needle and then co-cultured with Agrobacterium. The inoculated seeds can grow directly into plants and then set seeds without the process of tissue culture.
- In-situ dip flower method.
Workflow of rice transformation
- Vector construction
- Induction and subculture of rice recipient callus
- Agrobacterium-mediated rice genetic transformation
- Screen positive genetically modified seeds
- Differentiation of positive callus into shoots
- Strong plantlets and rootage of regenerated green seedlings
- PCR detection of foreign genes in T0 generation transgenic seedlings
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information
- Some pictures of genetic transformation and screening
- Picture of some samples of callus differentiation and regeneration
- Agarose gel electrophoresis image of foreign gene PCR detection of T0 generation transgenic seedlings
- T0 generation transgenic vaccine: If we use the vector and recipient provided by us, the T0 generation positive vaccine for each transformation experiment is ≥12; if the customer needs to use other vectors and recipients, we usually only promise to get the T0 generation positive vaccine 6-8 strains.
Service process
Online order — Confirm order / experimental material — Conduct experiment — Make a report — Express genetically modified seeds or seedling according to customer requirements
Project cycle
It takes about 5-6 months.
Advantages
- High transform efficiency
- T-DNA integration copy number is small
- Professional technology platform, the technical team engaged in plant genetic transformation for many years guarantees the success rate of the experiment
- Efficient experimental process
- Optimized mature embryo transformation system
- Not limited by materials
- Ready to carry out genetic transformation projects
- Perfect service support. Provide professional technical consultation and guidance in the early stage to optimize the project; communicate at any time during the experiment to keep abreast of the progress; after the project is completed, provide complete results according to the contract.
※ It should be noted that our service is only used for research.
Online Inquiry
