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Plant CNV Analysis Service

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Introductions

CNV Analysis

Copy number variation (CNV) is caused by the rearrangement of the genome. It generally refers to the increase or decrease in the copy number of large genome fragments with a length of more than 1 kb, which is mainly manifested as deletions and duplications at the submicroscopic level. CNV mainly affects gene expression, changes the structure of gene products, and even produces new fusion genes to participate in the process of plant growth and development and resisting adversity/disease. Since CNV is an important part of genome structural variation (SV), its mutation rate is much higher than SNP (Single nucleotide polymorphism), which can determine the variation of important economic traits of crops. Therefore, CNV is widely used in the field of agricultural molecular breeding, promoting the development of breeding selection and serving crop genetic breeding.

Services

With years of rich experience, Lifeasible provides customers with topic design, primer and probe synthesis, detection system optimization, specimen testing services for the research of gene CNVs, and provides customers with relevant bioinformatics analysis based on the final test results to help find and verify the CNVs Individual differences, phenotypic correlation, etc.

Research methods

Genome-wide CNV research methods Single-molecule candidate CNV research methods
  • Array-based comparative genomic hybridization (aCGH)
  • SNP sub-type chip technology
  • Next-generation sequencing technology
  • Fluorescence in situ hybridization (FISH)
  • Southern bloting
  • Competitive PCR
  • Fluorescence real-time quantitative PCR
  • Multiplex ligation-depengent probe amplification (MLPA)

Sample requirements

  • Fresh tissue > 1g
  • DNA ≥ 2 μg, concentration ≥ 50 ng / μl.

Project cycle

  • The experiment cycle of the project is different depending on the selected method, please consult our technicians for details.

Feedback to customers

  • A project report, including experimental procedures, experimental data, analysis results, etc.

Service process

  • Submit the relevant requirements and materials of the project.
  • Discuss project details with our professional technical team.
  • Revise the experimental plan based on the opinions after discussion.
  • Both parties are satisfied and reach an agreement to sign a technical service contract.
  • Carry out experiments and complete the content stipulated in the contract on time.
  • After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.

CNV Analysis

Advantages

  • Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
  • Advanced equipment, mature and stable methods.
  • Optimized experimental procedures ensure extremely high efficiency and specificity.
  • The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
  • Personalized customized package service.

※ For research or industrial use.

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