Overexpression of Plant Genes

INQUIRY

Introductions

Gene overexpression refers to cloning a target gene into a vector carrying elements such as a strong promoter and resistance selection markers; then introducing it into a plant, so that the host cell will obtain a higher level of target mRNA transcription and protein expression; so the function of the gene can be studied through phenotypic analysis. It is suitable for cases where gene functions are redundant or lethal after gene knockout.

Plant transformation refers to the transfer and incorporation of engineered genes or plasmids into the plant genome. It is not only an indispensable biotechnology method for in-depth study of plant growth, but also a promising tool for modern agriculture, especially a tool for introducing and selecting desired crop traits. Some of the most popular plant transformation methods include gene gun delivery, heat / electric shock, microinjection, virus / Agrobacterium / polyethylene glycol (PEG)-mediated transformation, protoplast fusion and silicon carbide fiber-mediated transformation. The choice of method depends on the target species and the specific research purpose.

Services

Lifeasible has developed a very powerful plant transformation platform that can produce transgenic plants. We provide transient and stable genetic transformation services for a variety of plant species, including model plants, major food crops, economic plants, etc.

Service content

• Arabidopsis thaliana
• Oryza sativa
• Zea mays
• Triticum aestivum
• Medicago truncatula
• Glycine max
• Gossypium hirsutum
• Nicotiana tabacum
• Solanum lycopersicum
• Brassica napus
• Solanum tuberosum

※ The project is constantly updated, please contact our staff by email for more up-to-date and relevant information.

Customer provided

• Target gene sequence or ready-made vector
• Target species name
• Uncommon plant species material
• Uncommon plant transformation vector

Feedback to customers

• Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information
• More than 6 independent positive lines
• PCR verification of genome of transgenic plants

Service process

• Communicate with customers
• Design experiment
• Sign contracts and confidentiality agreements with customers
• Update project status
• Organize experimental data and pictures, analyze the results and make a report

Service description

• Customers need to provide information about the target gene and screening resistance.
• Customers need to confirm genetically transformed varieties.
• We do not accept lethal or severely defective gene transformation services. If it is possible to cause death, please inform our company in advance.
• Due to the uncertainty of biological experiments, the company will normally charge part of the basic experiment fees if the company's operations are correct but the conversion fails.

Advantages

• Mature plant transgenic technology system.
• It can provide transgenic services for many species such as Arabidopsis, rice, wheat, tomato, corn, cotton, soybean, tobacco, rape and other species. 
• Professional team, rich experience.
• Quality assurance, efficient service.
• Standardization system, scientific management.
• Personalized customized package service.
• With modern equipment, experienced team, cutting-edge technology.

 

※ It should be noted that our service is only used for research.

Related Sections

• Plant Genome Editing with CRISPR / Cas9
• Plant Genome Editing with TALEN
• Plant Genome Editing with ZFN
• RNAi Mediated Plant Gene Silencing
• Virus-Induced Gene Silencing (VIGS) of Plant Genes

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