Plant Circular RNA Sequencing

INQUIRY

Introductions

Circular RNA (circRNA) is a special type of non-coding RNA molecule, and it is also the latest research hotspot in the RNA field. Different from traditional linear RNA (linear RNA, containing 5'and 3'ends), circRNA molecules are in a closed loop structure, which is not affected by RNA exonuclease, and their expression is more stable and not easily degraded. Some circRNAs are rich in microRNA (miRNA) response elements (MRE), which can play the role of competitive endogenous RNA, bind to miRNAs, and act as miRNA sponges in cells, inhibit miRNAs from functioning and regulate gene expression levels. Circular RNA also has temporal and spatial specificity and evolutionary conservation. These characteristics show that circRNA may play an important role at the transcriptional and post-transcriptional levels. Circular RNA sequencing is more and more widely used in the fields of medicine and agronomy.

Services

Lifeasible uses illumina sequencing platform to perform circular RNA (circRNA) sequencing on samples with reference genomes, and carry out accurate circRNA identification and source gene analysis.

Workflow of circular RNA sequencing

Analysis content

• Reference sequence alignment
Analyze the comparison rate and coverage of sequencing reads
• circRNA identification
Identify the circRNA present in the sample
• circRNA source gene analysis
Help clarify which genes can form circRNA
• CircRNA expression level quantification
Calculate the expression level of circRNA in each sample
• Differential expression analysis
CircRNA differentially expressed between samples
• GO / KEGG enrichment analysis
Clarify the specific biological function of the source gene
• miRNA binding site prediction
The basis of circRNA's sponge effect
• circRNAs-miRNAs-mRNAs association analysis

Sample requirements

• Total RNA≥15μg, RNA concentration≥100ng/μl, RIN≥7.5
• Please put the sample in a 1.5ml tube, indicate the sample name, concentration and preparation time on the tube, and use parafilm to seal the tube mouth.
• Before shipping, fix all sample tubes in 50ml centrifuge tubes with caps and ship them on dry ice.

Project cycle

• The entire project generally takes about 2 months.

Feedback to customers

• Raw data
• A project report includes experimental process, instrument parameters, analysis methods and results, etc.

Service process

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• Lifeasible enriches circRNA efficiently, uses mainstream software to identify circRNA, and analyzes source genes to deepen the functional research on circRNA of the project species.
• The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
• Personalized customized package service.

 

※ For research or industrial use.

Related Sections

• Eukaryotic Transcriptome Sequencing without Reference Genome
• Eukaryotic Transcriptome Sequencing with Reference Genome
• Prokaryotic Transcriptome Sequencing Analysis
• LncRNA Sequencing of Plant
• Plant Small RNA Sequencing
• Plant Comparative Transcriptome Service
• Plant Isoform-sequencing with Reference Genome
• Plant Isoform-sequencing without Reference Genome
• Ribo-seq of Plant
• Metatranscriptome Sequencing of Plant

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