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Breeding for High Quality and High Yielding Barley

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Why Breed High Quality and High Yielding Barley?

Barley, Hordeum vulgare, one of the major cereals planted in the world, is a founder crop of the early food production and one of the earliest crops domesticated. It is an important crop, ranking fourth in world crop production. Barley is used for animal feed, brewing malts and human food. Nowadays, food security has become a major issue, human population is expected to reach 10 billion by 2050. To meet the need to feed an increasing number of people, we need high quality and high yielding barley.

Breeding Services for High Quality and High Yielding Barley

Traditional breeding shows disadvantages such as uncontrollable, long cycle and more interference. Multiple quality and yielding related genes had been reported. Lifeasible provides numbers of methods to accelerate the breeding process of high quality and high yielding barley.

  • QTL Mapping: A candidate gene approach which is performed in constructed bi-parental populations with contrasting parents to detect various QTLs and then estimate its position and effect on the plant traits after introgression.
  • Gene Mapping: A method that the genetic markers are developed and a population is mapped to identify the distances between genes via analysis of co-segregation patterns and specific locus of a gene based solely on their phenotypic effect.
  • Marker-assisted Selection: A type of indirect selection for the desired plant phenotype which is based on the linked molecular markers banding pattern.
  • Tilling by sequencing (Tbys): A general reverse genetics strategy which includes mutagenesis of a large plant population followed by identification of point mutations in the gene of interest via high-throughput detection system.
  • Transgenics: biotechnological interventions, including genetic engineering, RNA silencing technologies, and gene editing.

Barley adaptation to local environmentsFigure 1. Barley adaptation to local environments. (Maeda, A.E.; et al., 2022)

Transgenic Work Flow

  • Construction of transformation vector
  • Prepare explants
  • Genetic transformation of barley
  • Identification of resistant plant
  • Access to transgenic barley
  • Delivery of reports and plant materials

Advantages of High Quality and High Yielding Barley Breeding Service

  • Barley specific culture medium and transformation procedures to ensure fast and accurate transformation.
  • Extensive SOP operation specification and multiple experimental quality control (QC) procedures to improve transgenic efficiency.
  • Efficient transgenic system to obtain transgenic plants quickly.
  • Provide complete, comprehensive and accurate reports of the item.

Lifeasible is committed to accelerating the selection of barley varieties with high quality and high yield to shelf through the molecular biology technology platform. If you have relevant research needs or want to learn more about our services, please do not hesitate to contact us.

Reference

  1. Maeda, A.E.; et al. Plant clock modifications for adapting flowering time to local environments, Plant Physiology. 2022, 190(2): 952–967.

※ It should be noted that our service is only used for research.

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