Smartseq2 Single Cell Transcriptome Sequencing of Plant

INQUIRY

Introductions

Smart-seq2 single-cell transcriptome sequencing uses SMART amplification technology to perform reverse transcription and PCR amplification of mRNA in a single cell, combined with transposase library building technology, and finally uses high-throughput sequencing to analyze gene expression quantification, functional enrichment, and metabolic pathways of mRNA in single cells. It can solve the problem of very low sample volume or cell heterogeneity in traditional RNA quantification technology. It is a powerful tool for studying gene expression at the single-cell level and greatly expands the scope of RNA-Seq applications. This technology has been widely used in the field of agricultural molecular breeding, such as research on insect resistance, virus resistance, herbicide resistance, and stress resistance of plants, research on improving seed storage protein, research on fruit maturity, and research on cytoplasmic male Childbearing and self-incompatibility, etc.

Services

With years of rich experience, Lifeasible provides smartseq2 single cell transcriptome sequencing and analysis service. Customers only need to provide cells, tissues or RNA, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.

Workflow of smartseq2 single cell transcriptome sequencing

Analysis content

Standard analysis	Statistics and quality control of raw sequencing data
	Align reference genome or reference gene sequence
	Sequencing and comparison evaluation
	Gene expression statistics
	Transcript prediction and annotation of new genes (reference genome is required)
	Sample relationship analysis
	Differentially expressed gene analysis (1) Differentially expressed gene screening (2) Cluster analysis of differential gene expression patterns (3) Significant enrichment analysis of GO function of differential genes (4) Significant enrichment analysis of pathway of differential genes
Advanced analysis	SNP analysis
	Gene structure optimization (reference genome is required)
	Variable splicing identification (reference genome is required)

Sample requirements

• Cell

1-500 single cells (can detect 1~10,000)

• Micro RNA

10pg~100ng purified total RNA with poly A sequence

• RNA quality

28S/18S≥1, RIN≥7, concentration>50pg/μl

• It is recommended to prepare no less than 4 duplicate samples for each cell sample

Project cycle

• The entire project generally takes about 3 months.

Feedback to customers

• Raw data
• A project report includes experimental process, instrument parameters, analysis methods and results, etc.

Service process

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
• Strict quality control is carried out on each key step of the experiment, and the quality of the experiment is monitored throughout the process to ensure that customers receive high-quality data.
• With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

Related Sections

• 10x Genomics Single Cell Transcriptome Sequencing of Plant
• 10x Genomics Single Cell ATAC Sequencing of Plant
• 10x Genome Sequencing of Plant

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