10x Genomics Single Cell Transcriptome Sequencing of Plant

INQUIRY

Introductions

10x Genomics single cell transcriptome sequencing (single cell RNA-seq, scRNA-seq) is a new technology for high-throughput sequencing of mRNA at the single-cell level. It can study single cells with high-throughput and single-molecule resolution. The expression profile of cells reveals the heterogeneity of complex cell populations, and prevents the gene expression signals of individual cells from being obscured by population averaging. It brings brand-new methods and convenient means for cell type identification, signal pathway differential response, molecular regulatory network, and cell heterogeneity research. Single-cell transcriptome technology has been widely used in the field of agricultural molecular breeding, such as studying the classification of cell subgroups, identifying rare cell types, studying pathological diagnosis, screening specific molecular markers, and so on.

Services

With years of rich experience, Lifeasible provides 10xGenomics single cell transcriptome sequencing and analysis service. Customers only need to provide tissues or cell suspension, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.

Workflow of 10xGenomics single cell transcriptome sequencing

Analysis content

Sample requirements

• Sample type

Prepared cell suspension. The tissue needs to be dissociated into a single cell suspension, and plant cells need to be made into protoplasts.

• Cell viability

More than 90% of living cells

• Cell size

Less than 40μm

• Cell culture media and buffers should not contain substances that affect enzyme activity such as Ca²⁺ and Mg²⁺. 

Project cycle

• The entire project generally takes about 2 months.

Feedback to customers

• Raw data
• A project report includes experimental process, instrument parameters, analysis methods and results, etc.

Service process

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
• Strict quality control is carried out on each key step of the experiment, and the quality of the experiment is monitored throughout the process to ensure that customers receive high-quality data.
• With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

Related Sections

• Smartseq2 Single Cell Transcriptome Sequencing of Plant
• 10x Genomics Single Cell ATAC Sequencing of Plant
• 10x Genome Sequencing of Plant

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