Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Ribo-seq of Plant
INQUIRYIntroductions
Ribo-seq is the abbreviation of ribosome profiling sequencing, which is a major technical means to study translationomics by using sequencing methods. Translation group sequencing refers to the sequencing of RNA fragments that are being translated combined with ribosomes to accurately obtain information and accurate quantification of all translatable molecules (including mRNA and other potentially translatable RNA molecules such as lncRNA, circRNA, etc.) in the sample. It is the bridge between the transcriptome and the proteome. It can analyze differentially expressed genes in the process of life at the translation level, such as environmental stimulation, metabolism or disease that affect translation efficiency, RNA structure or post-transcriptome modification affects translation products, opening a new perspective for scientific research. Its application fields are also very wide, such as studying the regulation mechanism of animals and plants under adversity stress, studying the molecular mechanism of ontogeny and senescence, and so on.
Services
With years of rich experience, Lifeasible provides ribosome profiling sequencing and analysis service. Customers only need to provide cells, tissues or RNA, and we complete the entire service process from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of Ribo-seq
Analysis content
| Standard information analysis |
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| Advanced information analysis (association analysis with transcriptome) |
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| Customized analysis |
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Sample requirements
- Cell samples≥1×106. Cell samples need to be pre-treated with Harringtonine and cycloheximide, and then sent by quick freezing in liquid nitrogen.
- Plant tissue samples≥400mg. After the tissue samples are collected, they are quickly frozen in liquid nitrogen and transported on dry ice.
Project cycle
- The entire project generally takes about 3 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
- Strict quality control is carried out on each key step of the experiment, and the quality of the experiment is monitored throughout the process to ensure that customers receive high-quality data.
- With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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