Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant SNP Analysis Service
INQUIRYIntroductions
SNP (Single Nucleotide Polymorphisms) refers to DNA sequence polymorphisms caused by single nucleotide variations at the genome level. There are many such genetic markers and rich in polymorphism. Including single base conversion, transversion, insertion and deletion, etc. The distribution of SNPs in a single gene, the entire genome, and among populations is uneven. Generally speaking, SNP refers to a single nucleotide variation with a variation frequency greater than 1%. SNP research is mainly divided into SNP discovery and SNP genotyping. ①Analyze unknown SNPs, search for unknown SNPs or determine the relationship between an unknown SNP and a phenotype. After discovering the DNA strands containing SNPs, they are sequenced to determine the location of the mutation and the type of base. ②Analyze the known SNPs and detect the genetic diversity of SNPs in different populations. SNP is an important basis for studying the genetic variation of plant strains, and it can perform fine mapping of trait genes, molecular-assisted breeding, and seed resource identification, so it is widely used in agricultural molecular breeding.
Services
With years of rich experience, Lifeasible provides SNP analysis service in plant. We provide a one-stop service model. As long as you provide experimental samples, we will complete a series of processes from sample extraction to data analysis.
Analytical method
| KASP |
| Direct sequencing |
| TaqMan probe |
| SNPSHOT |
| MassARRAY |
Sample requirements
- Fresh tissue > 1g
- DNA ≥ 2 μg, concentration ≥ 50 ng / μl, OD260/280 = 1.7~1.9, no PCR inhibitor.
Project cycle
- The experiment cycle of the project is different depending on the selected method, please consult our technicians for details.
Feedback to customers
- SNPs results (Excel spreadsheet)
- Original SNP peak pattern
- Complete experiment report, including amplification and reaction system, primer sequence, probe sequence, etc.
- Other relevant information required by the customer
Service process
- Submit the relevant requirements and materials of the project.
- Discuss project details with our professional technical team.
- Revise the experimental plan based on the opinions after discussion.
- Both parties are satisfied and reach an agreement to sign a technical service contract.
- Carry out experiments and complete the content stipulated in the contract on time.
- After the project experiment is over, we will provide the original experimental data, analysis results, experimental procedures, experimental conditions, etc.
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- Advanced equipment, mature and stable methods.
- Optimized experimental procedures ensure extremely high efficiency and specificity.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Personalized customized package service.
※ For research or industrial use.
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