Solutions
- Molecular Breeding Solutions of Citrus
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- Breeding of Paeonia
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- Breeding of Petunia hybrida
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- Breeding of Cut Flowers
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- Molecular Breeding Solutions of Tobacco
- Molecular Breeding Solutions of Vegetables
- Molecular Breeding Solutions of Medicinal Plant
- Molecular Breeding Solutions of Flowers
Breeding of Insect- and Herbicide-resistant Tobacco
INQUIRYIntroduction
Tobacco is an important crop in many parts of the world, with the advantages of large yield, wide adaptability and high economic value. Especially in recent years, tobacco has been widely used as a model plant in laboratories. The large-scale planting of tobacco can greatly promote the development of local economy and improve the ecological environment.
Tobacco is susceptible to pests, which can cause poor tobacco growth and affect the quality of the tobacco leaves. Common pests in tobacco include silkworms, locusts and aphids. Weeds will greatly affect the yield and quality of tobacco, and herbicides will cause obvious damage to crops while removing weeds, so it is particularly important to obtain herbicide-resistant crops.
Through molecular breeding, tobacco varieties with some resistance to these herbicides and pests can be selected to improve tobacco yield and quality.
Service
As a model plant, tobacco has developed a more mature system for selecting resistant plants using molecular biology techniques. Crop resistance to pests and various herbicides can be improved through genetic engineering targeting resistance genes. Lifeasible offers several strategies to accelerate the breeding process for pest and herbicide resistant tobacco.
- Sequencing-based breeding: High-throughput sequencing technology allows comprehensive study of crop genomes, cloning and study of genes controlling insect and herbicide resistance traits, and mastering genetic patterns.
- Plant genetic engineering: Plant genetic engineering technology can knock out, silence, over express target genes or transfer foreign genes.
- Construction of transformation vector: Transfer of pest or herbicide resistant gene into vector.
- Preparation of explants: Leaves, stems or seeds can be selected as explants, then sterilize and put it into appropriate culture medium for aseptic culture.
- Genetic transformation of tobacco: Get transgenic tobacco by Agrobacterium tumefaciens mediated explant method.
- Identification of resistant plants: Screening of transgenic tobacco with corresponding antibiotic resistance.
- Genetic marker assisted breeding: For phenotypes with inaccurate genetic measurement of some traits, genetic markers such as cytological and biochemical markers can be used for assisted selection, which can speed up the breeding process.
- Access to large quantities of transgenic tobacco: Obtaining a large number of transgenic tobacco by tissue culture of resistant calli.
Figure 1. Agrobacterium mediated method. (Sheludko, Y. V. 2008)
Technology Platforms
- Genetic engineering technology platform: CRISPR / Cas9, RNA Interference (RNAi), gene overexpression.
- Genetic transformation service platform: High-level transgenic and gene editing technology, fast and efficient genetic transformation.
- Vector construction platform: Rich vector resources can provide vector with different resistances to meet diversified needs.
- High-throughput sequencing technology platform: DNA-level sequencing, RNA-level sequencing, single cell level sequencing, epigenetics level sequencing, proteomics and metabolomics services.
Advantages
- Experienced technical team and standardized experimental platform to meet diversified needs
- Provide personalized professional services for different customer needs
- Communicate with customers in a timely manner to keep you informed of the progress of experiments
- Timely resolution of customer concerns and effective solutions
- One-stop service & high efficiency to ensure obtaining transgenic plants in a short time
Lifeasible is committed to helping you obtain transgenic plants in a short period of time through molecular biology techniques. If you need more information about related research and services, please feel free to contact us and we will be glad to reply to you.
Reference
- Sheludko, Y. V. Agrobacterium-mediated transient expression as an approach to production of recombinant proteins in plants. Recent Patents on Biotechnology. 2008, 2(3): 198-208.
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