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- Molecular Breeding Solutions of Citrus
- Molecular Breeding Solutions of Rice
- Molecular Breeding Solutions of Wheat
- Breeding for Trait Improving Ornamental Flower
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- Spores Breeding Solutions
- Molecular Breeding Solutions of Potato
- Breeding of Ornamental Flower
- Breeding of Cut Flowers
- Breeding of Carnations
- Breeding of Dianthus caryophyllus L.
- Breeding of Paeonia suffruticosa
- Breeding of Lilium
- Breeding of Chrysanthemums
- Breeding of Eustoma grandiflorum
- Breeding of Anthurium andraeanum
- Breeding of Rose
- Breeding of Paphiopedilum
- Breeding of Gerbera hybrida
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- Breeding of Alstroemeria aurea Graham
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- Breeding of Pelargonium
- Breeding of Rhododendron
- Breeding of Oncidium
- Breeding of Cymbidium goeringii
- Breeding of Dendrobium officinale
- Breeding of Hippeastrum striatum
- Breeding of Japanese Gentians
- Breeding of Viola tricolor L.
- Breeding of Jasmine
- Breeding of Polianthes tuberosa L.
- Breeding of Schlumbergera truncata
- Breeding of Ornithogalum
- Breeding of Ruta graveolens L.
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- Breeding of Lonicera japonica
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- Breeding of Paeonia
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- Breeding of Cut Flowers
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- Molecular Breeding Solutions of Flowers
Spores Breeding Solutions for Pteridophytes
INQUIRYIntroduction
Pteridophytes are the world's only seedless vascular plants and represent a transition between bryophytes and seed plants. Lacking seeds, pteridophytes reproduce nutritionally through spores. Most pteridophytes are homosporous, producing only single-sized spores. Homosporous pteridophytes have a unique free-living gametophyte among vascular plants and may be hermaphroditic. Compared to all heterosporous plants (including seed plants), these plants have unusual life cycles of evolutionary importance, including:
(i) The two free-living organisms, gametophytes and sporophytes, often have different ecological requirements.
(ii) Both gametophytes and sporophytes have their selective forces.
(iii) Self-fertilization of both sexes' gametophytes produces pure sporophytes within one generation.
The spores produced by pteridophytes play an essential role in their dispersal and reproduction. Therefore, it is crucial to study pteridophyte spores' type, size, structure and morphological characteristics. In addition, information on the spore genome size or ploidy of pteridophytes is essential to determine their reproductive pattern.
Solutions
Pteridophytes form an important component of many habitats and ecosystems worldwide. Spores are not only a reproductive tool for fern flora but also an important basis for fern species identification. Lifeasible has been working on the biological characteristics of pteridophytes spores for many years. We not only characterize the structure of pteridophytes spores but also provide solutions for the population biology of homosporous pteridophytes, including:
- Characterizing the genetic structure of homosporous spore pteridophytes populations.
- Identifying the genetic structure of homosporous spore pteridophytes species.
- Analysis of the genetic characteristics of spore pteridophytes in a conspecific population.
Genome size comparison between spores and leaves is a reliable and easy way to determine their reproductive patterns compared to traditional methods. Our botanists have summarized the current extensive genetic database of conspecific pteridophytes to provide a comprehensive fern spore breeding service. Here, we develop a customized process for assessing pteridophytes spore genome size using flow cytometry.
(1) Optimization of bead vortex conditions to extract spore nuclei from different lineages of pteridophytes.
(2) We use spore flow cytometry to infer their haploid genome size.
(3) We use estimated spore and leaf nucleus DNA content to determine reproductive fern patterns.
Advantages
- Genetic data were obtained by well-established enzyme electrophoresis.
- Extracting spore nuclei takes <1 minute, and multiple samples can be processed together.
- Only commonly used laboratory equipment is required for completion.
- The highest quantity and quality of spore nuclei can be obtained.
- An effective method for determining pteridophytes reproduction patterns.
- Comprehensive assessment of characteristics such as shape, type, and size of fern spores.
- Cutting-edge molecular genetic methods to analyze polyploidy, breeding systems, and genetic differentiation in homosporous pteridophytes.
Lifeasible's spores breeding solutions for pteridophytes are widely used in molecular genetics studies of pteridophytes spore germination and breeding. Our highly skilled and dedicated scientific staff ensures that the most suitable methods and techniques are selected for each dedicated spore breeding project. If you have any special requirements for our solutions, please feel free to contact us. We look forward to working with you on attractive projects.
Reference
- Soltis D E, Soltis P S. Polyploidy, breeding systems, and genetic differentiation in homosporous pteridophytes[M]//Isozymes in plant biology. Springer, Dordrecht, 1989, 241-258.
※ It should be noted that our service is only used for research.
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