Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
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- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
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- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
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- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
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- Plant Comparative Transcriptome Service
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- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
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- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
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- Plant Tissue and Cell Imaging Services
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- Genetically Modified Plant Testing Services
- Seed Testing Services
Triticum aestivum Transformation
INQUIRYIntroductions
Wheat is a crop of the monocotyledonous plant, Gramineae, and Triticum genus. The Latin name is Triticum aestivum. It is one of the most important crops in the world, with great nutritional value and high economic value. It is a hexaploid crop with a complex genetic background and a relatively large genome (about 16,000 Mb), which is 7 times that of maize and 35 times that of rice. In recent years, in order to strengthen the research on wheat genes with important utilization value, a large amount of gene sequence information has been collected on a large scale. Researchers use this genetic information to carry out genetic transformation and identify key genes with breeding value, especially those related to agronomic traits such as high yield, high quality, resistance to pests and diseases, and stress resistance. It provides important genetic resources for wheat molecular breeding.
Services
Lifeasible has established a Triticum aestivum transformation platform through Agrobacterium-mediated method and biolistic-mediated method. The platform has a complete, large-scale, standardized technical system, and provides professional and convenient technologies for wheat genetic transformation for agricultural personnel who serve wheat as the research object. We can provide customers with wheat genetic transformation services, including experimental design, vector construction, plasmid transformation, positive transplantation screening and testing, etc. We have an experienced team that can help you solve technical problems and challenges in wheat conversion to help your project succeed.
Customer provided materials
- The sequence of the target gene and the Gene Bank ID
- Plasmids, Agrobacterium, etc.
- Vector to be transformed (conventional vector can also be provided by us)
- Unconventional receptor material
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Receptor material
- Recipient material varieties: Zhengmai 9023, Kenong199, Longchun23, Bobwhite, Fielder, Westonia, Gascoigne, Sunvale, Sunstate, Stewart, Gladius, Janz, etc.
- Recipient material types: immature embryos, young ears, mature embryo.
Transformation methods
- Particle bombardment of shoot apical meristems (SAMs) grown from dry imbibed seeds.
- The embryo apical meristem of the mature seeds after soaking is punctured with a needle, and then co-cultured with Agrobacterium.
- Infiltration of wheat spikes at the early boot stage with Agrobacterium.
Workflow of wheat transformation
- Vector construction
- Induction and subculture of wheat recipient callus
- Agrobacterium-mediated or biolistic-mediated of wheat genetic transformation
- Screening
- Differentiation of positive callus into shoots
- Strong plantlets and rootage of regenerated green seedlings
- PCR detection of foreign genes in T0 generation transgenic seedlings
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information
- Some pictures of genetic transformation and screening
- Picture of some samples of callus differentiation and regeneration
- Agarose gel electrophoresis image of foreign gene PCR detection of T0 generation transgenic seedlings
- T0 generation transgenic vaccine: If we use the vector and recipient provided by us, the T0 generation positive vaccine for each transformation experiment is ≥12; if the customer needs to use other vectors and recipients, we usually only promise to get the T0 generation positive vaccine 6-8 strains.
Service process
Project cycle
It takes 5-6 months from the construction of the vector to the test results of the T0 generation transgenic seedlings. And experiments can be carried out throughout the year.
Advantages
- We provide two systems for your choice: Agrobacterium-mediated method and gene gun bombardment method.
- Both of these transformation methods can transform callus obtained from a variety of explants, such as freshly isolated immature embryos, pre-cultured immature embryos, or embryogenic callus extracted from mature or immature embryos.
- Low genotype dependence
- Not limited by materials
- High transform efficiency
- High efficiency of wheat regeneration system
※ It should be noted that our service is only used for research.
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