Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
RNAi Mediated Plant Gene Silencing
INQUIRYIntroductions
Gene silencing is an important means of regulating gene expression in eukaryotic cells. It refers to a mechanism that is induced by double-stranded RNA in eukaryotes to recognize and eliminate abnormal RNA in cells. It is an evolutionarily conservative defense against transgenic or foreign viruses. RNA interference (RNAi) phenomenon introduces double-stranded RNA (dsRNA) containing the homologous complementary sequence of the target gene mRNA into the cell, which can specifically recognize the mRNA, cause the degradation of the mRNA, and cause the corresponding loss of function. RNAi provides an economical, fast, and efficient technical means to inhibit the expression of specific genes. This technology has been widely used. At present, the commonly used means of RNA interference are miRNA, shRNA and siRNA. The rapidity and universal affordability of RNAi have made cutting-edge progress in plant engineering and crop improvement, leading to the discovery of new crops with enhanced functions, such as nicotine-free tobacco, non-allergic peanuts, decaffeinated coffee and nutrition Fortified corn.
Phenotype comparison after RNAi-mediated gene silencing in Arabidopsis (Weng J K et al., 2010)
Services
Lifeasible has established RNAi-mediated gene silencing systems, which can be conveniently and effectively used for plant gene silencing and phenotypic modification. It can complete the design of multiple sequences of siRNA / miRNA, and provide comprehensive RNAi solutions from siRNA synthesis, RNAi vector construction to RNAi function verification. We uphold the customer-centric tenet and are committed to providing better services, saving customers a lot of time and energy.
Service content
- siRNA design and vector construction
- Transgenic plant
- Phenotype and gene function analysis
- Make a report
Design primers - Construction of siRNA stem-loop structure - Construct the vector - Sequencing verification
Fluorescence quantitative PCR detection / mRNA expression detection / Western Blot protein expression detection
Sequencing report of constructed vector, fluorescent quantitative PCR test report, Western Blot picture, etc.
Feedback to customers
- siRNA information
- Sequencing report of constructed vector
- The plasmid and bacteria of the constructed vector
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information
- Phenotype and gene function analysis pictures (customized according to requirements)
Service process
Service requirements
- Target gene sequence or accession number
- Required promoters and screening genes
Project cycle
- Takes about 2~3 months
Advantages
- Strong inhibitory effect in plants
- Long-term effective interference effect
- We have a wealth of experience, and the plants that have been verified are: Arabidopsis, rice, day lily, sweet potato, corn, etc.
- According to the source of the target gene, you can choose to construct an RNAi vector with 35S or Ubi promoter.
- Mature plant transgenic technology system
- With modern equipment, experienced team, cutting-edge technology
Reference
- Weng J K, Akiyama T, Bonawitz N D, et al. Convergent Evolution of Syringyl Lignin Biosynthesis via Distinct Pathways in the Lycophyte Selaginella and Flowering Plants[J]. The Plant Cell, 2010, 22(4):1033-1045.
※ It should be noted that our service is only used for research.
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