Post-translational Modification Proteomics (PTMs) Service for Plant

INQUIRY

Introductions

Protein post-translational modifications (PTMs) refer to the process of chemical modification of proteins during or after translati`on. Post-translational modification of proteins increases the functional diversity of the proteome by adding functional groups such as phosphate, acetate, amide or methyl groups to the protein. PTMs usually include phosphorylation, glycosylation, ubiquitination, nitrosylation, methylation, acetylation, lipidation, proteolysis and others. Post-translational modifications of proteins play a key role in many cellular processes, such as cell differentiation, protein degradation, signal transduction and regulatory processes, gene expression regulation, and protein interactions. It also participates in various physiological and pathological processes, such as regulating cell proliferation, development, differentiation, apoptosis and other life activities. Therefore, it is widely used in stress resistance, autophagy, cell cycle, signal pathways, circadian rhythm, growth and development and other research fields.

Services

Lifeasible uses an advanced mass spectrometry platform, combined with an advanced analysis platform, to provide post-translational modifications identification, such as phosphorylation / glycosylation / ubiquitination / acetylation / methylation / disulfide bond / nitrosylation for the majority of researchers Identification.

PTMs analysis service project

• Phosphorylation
• Ubiquitination
• Glycosylation
• Lipidation
• Methylation
• Proteolysis
• Acetylation
• Succinylation
• Propionylation
• Glutarylation
• Malonylation

※ The project of service content is constantly updated, please contact us for more up-to-date and relevant information.

Workflow of protein post-translational modification proteomics (PTMs)

Analysis content

Standard information analysis	Data output statistics
	Modified protein database establishment and search
	Modified protein identification
	Modified protein annotation
	Modified protein quantitative analysis
	Modified protein differential expression analysis
	Differentially modified protein GO/KEGG function enrichment analysis
Customized information analysis	Modified peptide motif analysis (available for some species)

Sample requirements

• Fresh plant tissue > 6 g
• The total amount of protein in the protein solution 5~10 mg

The sample delivery volume for acetylation modification needs to be 10 times the above requirement.

Project cycle

• It takes 50~70 working days to complete the standard process.

Feedback to customers

• Raw data
• A project report includes experimental procedures, related mass spectrometry parameters, and standard analysis results.

Service process

Advantages

• Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
• The experiment adopts a carefully optimized experimental procedure, which has extremely high efficiency and specificity.
• The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
• With a strong bioinformatics team, it can meet the various in-depth data analysis needs of customers.
• Personalized customized package service.

 

※ It should be noted that our service is only used for research.

Related Sections

• Proteomics Qualitative Analysis in Plant
• Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
• Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
• Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)

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