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Plant Nucleic Acid Hybridization Detection

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Nucleic Acid Hybridization Detection

Nucleic acid hybridization is a technique based on base complementation, which uses the denaturation and renaturation of double-stranded nucleic acid molecules under specific conditions as a means to perform qualitative and quantitative detection of nucleic acid molecules. It transfers the nucleic acid fragments to be detected, which are produced by enzyme digestion, electrophoresis separation and denaturation, to a specific membrane, and hybridizes with a labeled probe with a known specific sequence to check whether the nucleic acid fragment is known probes have homologous sequences.

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Lifeasible has established a complete nucleic acid hybridization detection platform of plant to help you carry out relevant experimental services.

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Service process

Nucleic Acid Hybridization Detection -Lifeasible

Application of nucleic acid hybridization detection in agricultural molecular breeding

  • Perform a chromosome map analysis
  • Determine the copy number of a specific DNA sequence
  • Identify disease-related restriction fragment length polymorphism markers (RFLP)
  • Screening for gene clones
  • Detection of different concentrations of DNA
  • Identify the expression sites of specific genes
  • Perform preliminary analysis of RNA structure
  • Perform quantitative detection of specific RNA
  • Analyze changes in the content of gene transcription
  • End-labeled oligonucleotide probes can detect point mutations
  • Determine if there is a virus infection

Advantages

  • Nucleic acid hybridization technology has the characteristics of good specificity, high sensitivity, fast diagnosis, relatively simple operation, and high cost (radioisotope labeling).
  • Standardization system, scientific management
  • Complete layout of the biotechnology chain, seamless connection with downstream experiments
  • Leading technology platform conditions, first-class R&D and production equipment

※ For research or industrial use.

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