Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Nucleic Acid Hybridization Detection
INQUIRYIntroductions
Nucleic acid hybridization is a technique based on base complementation, which uses the denaturation and renaturation of double-stranded nucleic acid molecules under specific conditions as a means to perform qualitative and quantitative detection of nucleic acid molecules. It transfers the nucleic acid fragments to be detected, which are produced by enzyme digestion, electrophoresis separation and denaturation, to a specific membrane, and hybridizes with a labeled probe with a known specific sequence to check whether the nucleic acid fragment is known probes have homologous sequences.
Services
Lifeasible has established a complete nucleic acid hybridization detection platform of plant to help you carry out relevant experimental services.
Service content
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Southern Blot Hybridization
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Northern Blot Hybridization
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Dot Blot Hybridization
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In Situ Hybridization
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Gene Chip
Service process
Application of nucleic acid hybridization detection in agricultural molecular breeding
- Perform a chromosome map analysis
- Determine the copy number of a specific DNA sequence
- Identify disease-related restriction fragment length polymorphism markers (RFLP)
- Screening for gene clones
- Detection of different concentrations of DNA
- Identify the expression sites of specific genes
- Perform preliminary analysis of RNA structure
- Perform quantitative detection of specific RNA
- Analyze changes in the content of gene transcription
- End-labeled oligonucleotide probes can detect point mutations
- Determine if there is a virus infection
Advantages
- Nucleic acid hybridization technology has the characteristics of good specificity, high sensitivity, fast diagnosis, relatively simple operation, and high cost (radioisotope labeling).
- Standardization system, scientific management
- Complete layout of the biotechnology chain, seamless connection with downstream experiments
- Leading technology platform conditions, first-class R&D and production equipment
※ It should be noted that our service is only used for research.
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