Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Solanum lycopersicum Transformation
INQUIRYIntroductions
Tomato, whose scientific name is Solanum lycopersicum Mill., is an annual or perennial vegetable crop belonging to the Tubular order, Solanaceae, and Tomato genus. It is rich in nutrients and has a special flavor. It can be eaten raw or cooked, and processed in tomato sauce or tomato juice, also can be stored in whole fruit jars. As a representative of Solanaceae plants and climacteric fruits, tomato has the advantages of smaller genome, shorter growth cycle, self-pollination, clear genetic background, etc., and has a mature genetic transformation system. It has a relatively distant relationship with other model plants, such as Arabidopsis, rice, etc., so it has become one of the classic model plants for studying fruit development and maturation, and has important economic and scientific value.
Services
Lifeasible has established a tomato genetic transformation platform, using Agrobacterium-mediated method to provide large-scale and standardized transformation technology to realize tomato genetic transformation. Customers can provide target genes, plasmids, Agrobacterium, etc., and we will complete the entire experimental process from constructing vectors to obtaining transgenic vaccines.
Customer provided materials
- If only gene sequence information (or Gene Bank ID) is provided, our company can undertake gene amplification and vector construction services, which are charged separately.
- Construct a vector of Escherichia coli, plasmid, but provide clear resistance information for screening.
- Unconventional receptor material.
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Solanum lycopersicum transformation method
Agrobacterium-mediated transformation
Receptor material
- Recipient species
- Recipient types
Micro-Tom, Moneymaker, Ailsa Craig, LA178, vf36, LA1589, M82, AC, Heinz1706, Micro Tom, Rhubarb, Shangnong1734 etc.
Cotyledon
Hypocotyl
Workflow of tomato transformation by Agrobacterium-mediated method
Feedback to customers
- Complete experimental methods, original experimental records, instrument parameters, reagents and consumables information
- More than 10 independent positive lines
- PCR verification of genome of transgenic plants
- Pictures of explant infection, induced callus, embryogenic callus, tissue differentiation, plant regeneration, etc.
Service process
Project cycle
- It takes about 4 months.
Advantages
- It adopts Agrobacterium-mediated transformation, which is fast, efficient, stable, single-copy insertion, and is not limited by genotype.
- Professional technology platform. The technical team engaged in plant genetic transformation for many years guarantees the success rate of the experiment.
- Efficient experimental process. The optimized tomato genetic transformation system is not limited by materials, and genetic transformation projects can be carried out at any time.
- We have a variety of labeled vectors and media to provide you with a wide range of choices.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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