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Chestnut Yellow Crinkle Disease-Resistant Breeding

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Chestnut yellow crinkle disease is a leaf disease caused by a plant pathogen rapidly spreading in chestnut production areas, causing deep damage and widespread coverage. Chestnut yellow crinkle disease often causes the death of all chestnut trees in chestnut plantations, which seriously impacts the development of the chestnut industry. Lifeasible focuses on chestnut disease resistance breeding research. We provide from the chestnut yellow crinkle disease pathogen identification, to the chestnut resistance gene mining and gene function verification, molecular assisted breeding technology system establishment, and other comprehensive biotechnology services.

Symptoms of Chestnut Yellow Crinkle Disease

  • Leaf blades turn yellow, leaf blades become smaller, and at the top of each branch, the few leaves show the most obvious changes.
  • Leaf blades become wrinkled, as evidenced by folds between the lateral leaf veins, and the leaf blades become non-spreading. Leaf blades have distinct yellow margins, with the tips of the leaf blades being the most obvious.
  • The tree cannot bear fruit normally after the disease, which is manifested by small hanging fruit, small and wrinkled chestnuts, underdevelopment, and no commercial value.

Explore Our Services of Chestnut Yellow Crinkle Disease-Resistant Breeding

Establishment of molecular-assisted breeding technology system

We have established a targeted and efficient molecular-assisted breeding technology system. By analyzing the genetic variation patterns of multiple traits in chestnut and combining them with molecular marker technology, we have helped researchers provide reliable solutions to shorten the breeding cycle and accelerate the selection and breeding of varieties resistant to chestnut yellow crinkle disease.

Chestnut genetic transformation

We have established a stable and effective Agrobacterium-mediated genetic transformation method for chestnut. Using chestnut embryonic healing tissues of specific varieties, the embryonic healing tissues were co-cultured with Agrobacterium rhizogenes strains of specific plasmids to obtain embryonic healing transformed tissues, which could also be used to obtain transgenic chimeras. The Agrobacterium-mediated chestnut transformation system we established provides a basic platform for genetic improvement of core germplasm and further gene function validation.

Research on the pathogen-host interaction mechanism

We focus on , mining chestnut yellow crinkle disease resistance gene, and functional validation of important resistance genes. We have many successful cases of molecular interactions between phytoplasma and chestnut in gene (group) structure and function analysis, which provides theoretical support for chestnut disease resistance gene mining.
In addition, we also provide chestnut yellow crinkle disease pathogen identification services. Our technical team utilizes various highly sensitive molecular detection techniques, including PCR, real-time fluorescence quantitative PCR, and other technologies, which can significantly improve the efficiency and accuracy of pathogen detection.

Our Advanced Technology Platform

Genetic transformation platform Through transformation, we can realize the stable expression of exogenous genes and provide basic technology for chestnut gene editing.
Gene editing platform Utilizing targeted knockdown of specific genes to enhance regeneration efficiency and accelerate the creation of disease-resistant germplasm.
PCR technology platform Utilizing PCR amplification to improve detection sensitivity and for high-precision detection of phytoplasma-specific gene fragments.

Why Choose Us?

  • Strong technical team of experts.
  • A full range of technical support and solutions.
  • A perfect chestnut disease resistance breeding research technology platform.
  • Innovative and constantly optimized technology system.

Lifeasible specializes in breeding for resistance to important chestnut diseases, and our comprehensive solutions and advanced technical support are designed to help researchers in this field further accelerate the progress of chestnut disease resistance research. If you are interested in us, please feel free to contact us.

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