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Chestnut Internal Rot Disease-Resistant Breeding

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Chestnut internal rot is a disease that seriously affects the quality and yield of chestnuts. Its onset is caused by various pathogens, leading to mold and chestnut rotting during multiple periods. This reduces the edible value and commerciality of chestnuts and brings huge economic losses to chestnut growers and related enterprises, seriously restricting the development of the chestnut industry.

Lifeasible has rich experience in chestnut disease research. Relying on our advanced technology and professional technical team, we aim to provide various advanced technologies, including gene editing, to provide a powerful means to overcome chestnut internal rot disease resistance breeding research.

Symptoms of Chestnut Internal Rot Disease

Chestnut internal rot disease, the onset of chestnut seednuts produces black-gray, black, or dark green rot spots, and gradually becomes dry rot, appearing hollow, with gray-black mycelium clusters, seednuts are easy to crush. The diseased part is often infected by bacteria, turning into soft rot and producing a foul odor.

Explore Our Services of Chestnut Internal Rot Disease-Resistant Breeding

Molecular marker development and gene targeting services

  • Molecular marker screening for disease resistance. Based on the sequencing data of the chestnut genome, we have developed molecular markers closely linked to chestnut internal rot resistance genes and established an efficient molecular marker-assisted selection system.
  • Pathogen interaction gene mining. Through joint transcriptome and metabolome analyses, we analyze the key pathways of chestnut in response to pathogenic bacterial infestation, and provide targets for screening resistance-related functional genes.

Genetic transformation and gene editing services

  • Disease resistance gene mining and cloning. We utilize high-throughput sequencing, gene chip analysis, transcriptome sequencing, and other technological tools, which can help researchers to effectively excavate genes related to chestnut internal rot resistance and carry out cloning and functional verification.
  • Somatic cell embryogenesis and regeneration system. We have established an efficient in vitro regeneration system for chestnut, such as cotyledon or stem tip explant induction, and combined it with Agrobacterium-mediated genetic transformation technology to realize the introduction of disease resistance genes.
  • Gene editing breeding. We create new germplasm for disease resistance by designing gene editing programs for cloned disease resistance genes.

In addition, for chestnut internal rot resistance research, we also provide biological control and microbiome control technology for prevention and control. In addition, we will try to introduce exogenous disease-resistant genes into the chestnut genome by transgenic methods to develop highly resistant chestnuts against internal rot.

Our Advanced Technology Platform

Gene detection platform We are equipped with an advanced gene sequencer, a PCR instrument, a fluorescence quantitative PCR instrument, and other equipment, which can accurately and efficiently carry out DNA sequencing, gene expression analysis, molecular marker detection, and other work, and provide accurate gene detection data for disease resistance molecular breeding.
Genetic transformation platform We have established a perfect plant genetic transformation system, including various efficient gene transformation methods and rich transformation experience. This can realize the stable integration and expression of exogenous genes in chestnut and provide a solid technical guarantee for transgenic breeding.
Tissue culture and propagation platform We provide chestnut tissue culture and asexual propagation services, providing a large number of asexual materials for molecular breeding and ensuring the consistency and stability of breeding materials.

Why Choose Us?

  • We are a team of experts with expertise in chestnut disease resistance molecular breeding research, and can provide all-around technical support and solutions for our customers.
  • We have established an advanced molecular breeding technology platform for chestnut disease resistance, ensuring experimental data's accuracy and reliability.
  • We have accumulated rich project implementation experience and successful cases, which can help customers effectively reduce project risks and improve the project's success rate.

Lifeasible is committed to providing comprehensive solutions and advanced technical support for chestnut molecular disease resistance breeding research to help researchers advance their programs effectively. If you are interested in us, please feel free to contact us.

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