Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Small RNA Sequencing
INQUIRYIntroductions
Small RNA refers to endogenous non-protein RNA with a length of 18-30 nt, which is widely present in higher and lower organisms, and regulates the transcription and post-transcription level of mRNA and other life processes. Small RNA can be classified into three categories: miRNA (microRNA), siRNA (small interfering RNA), piRNA (piwi-interacting RNA). Small RNA controls gene expression by silencing and degrading its target gene molecules, and it plays an important regulatory role in the normal growth and development of organisms and the occurrence of diseases. This technology is mainly used in gene post-transcriptional regulation research, gene silencing research, small RNA target analysis and regulatory network and so on.
Services
Lifeasible adopts next-generation sequencing technology to provide a new and unique research platform suitable for in-depth identification and quantitative analysis of small RNAs of various species. Through in-depth sequencing, small RNA maps at the whole genome level of any species can be discovered, identified, and quantified, which advances the study of small RNA functions.
Workflow of small RNA sequencing
Analysis content
- Alignment reference sequence
- Classification notes for small RNA
- Base editing analysis
- Differential expression analysis
- Target gene prediction
- Differential sRNA target gene
Analyze the comparison rate and coverage of sequencing reads
Screen and identify known and new sRNA
miRNA sequence analysis
Differentially expressed sRNA between samples
sRNA target gene information
GO / KEGG enrichment, in-depth exploration of sRNA functions
Sample requirements
- Complete and pollution-free total RNA
- Small RNA (＜200nt)
- Please put the sample in a 1.5ml tube, indicate the sample name, concentration and preparation time on the tube, and use parafilm to seal the tube mouth.
- Before shipping, fix all sample tubes in 50ml centrifuge tubes with caps and ship them on dry ice.
Total RNA≥10μg, RNA concentration≥200ng/μl, RIN≥7.5, 28S/18S≥1.3
Small RNA total≥1μg, concentration≥20ng/μl
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- Lifeasible specializes in providing services related to agricultural molecular breeding, with standardized systems and scientific management.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
※ It should be noted that our service is only used for research.
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