Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Eukaryotic Transcriptome Sequencing without Reference Genome
INQUIRYIntroductions
Transcriptome refers to the collection of all transcripts transcribed from a specific cell or tissue of a species in a certain state. Transcriptome sequencing of eukaryotes without reference genomes can obtain reference sequences, and then quantitatively determine the changes in the expression level of each transcript during growth and under different conditions. Through next-generation high-throughput sequencing, it is possible to obtain almost all transcript sequence information of a specific tissue or organ of a certain species in a certain state in a comprehensive and rapid manner. This technology can mainly assist researchers for research purposes such as obtaining sample full transcript information, gene annotation and screening, and gene differential expression research. It has been widely used in various fields such as agriculture, medicine and food.
Services
With years of rich experience, Lifeasible provides eukaryotic transcriptome sequencing service without reference genome. We provide a one-stop service model. We complete a series of processes from sample extraction, quality testing, sequencing, biological information analysis to making reports.
Workflow of eukaryotic transcriptome sequencing without reference genome
Analysis content
Standard analysis
- Raw data filtering to remove linker sequences and low-quality reads
- Data output statistics and sequencing quality evaluation (sequencing saturation analysis, sequencing randomness analysis)
- Transcriptome denovo assembly
- Assembly quality statistics and analysis (contig N50, unigene length distribution, reads coverage statistics)
- Unigene expression statistics (gene coverage, expression, expression abundance distribution)
- Unigene basic function notes
- Unigene advanced feature notes
- SSR development and primer design
- Sample relationship analysis (principal component analysis, correlation coefficient heat map, sample cluster map)
- Differentially expressed genes analysis (two or more samples)
- Interaction network analysis
- GSEA analysis
- Mutation analysis (SNP/InDel analysis)
Advanced analysis
- Analysis of SMART protein domain database
- PlantTFDB plant transcription factor database analysis (plant samples)
- AnimalTFDB animal transcription factor database analysis (animal samples)
- PRGDB plant disease resistance gene database analysis (plant samples)
Sample requirements
- Total sample requirement≥5μg, concentration≥100ng/μl
- Animal sample purity RIN≥8, OD260/280≥1.8, 28S/18S≥1.0
- Please put the sample in a 1.5ml tube, indicate the sample name, concentration and preparation time on the tube, and use parafilm to seal the tube mouth.
- Before shipping, fix all sample tubes in 50ml centrifuge tubes with caps and ship them on dry ice.
Project cycle
- The entire project generally takes about 2 months.
Feedback to customers
- Raw data
- A project report includes experimental process, instrument parameters, analysis methods and results, etc.
Service process
Advantages
- From material selection, library construction and sequencing, to data analysis, every step has a scientific and meticulous design to ensure high-quality research results.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- The continuously updated and expanded high-performance computing platform ensures efficient data processing and secure data storage.
- Personalized customized package service.
- Standardization system, scientific management.
※ It should be noted that our service is only used for research.
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