Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Arabidopsis thaliana Transformation
INQUIRYIntroductions
Arabidopsis, also known as Arabian grass, is a cruciferous plant, and its Latin name is Arabidopsis thaliana (L.). Because Arabidopsis has the following characteristics, it has become an ideal material for genetic research:
- Self-pollinated, highly genetically homozygous
- Small genome（The total length of each haploid chromosome group (n=5) is only 70 million base pairs, that is, only 1/80 of the length of wheat chromosome group）
- Shorter plants
- Many seeds (each plant can produce thousands of seeds)
- Short growth cycle (about 4-6 weeks from germination to flowering)
- Planting conditions are easy to reach and planting is convenient
- Simple genetic transformation method
- It has obvious morphological characteristics, which is conducive to the observation of mutant phenotype.
Arabidopsis thaliana has been widely used in agricultural molecular breeding, for example, in the process of studying plant development, signal transduction, genetics, genomics, response to the environment, response to microorganisms, and so on.
Services
Lifeasible has established an Arabidopsis genetic transformation platform with Agrobacterium soaking method. The platform has a complete, large-scale and standardized technical system, providing professional and convenient technology for scientific research staff who service Arabidopsis as the research object.
Customer provided materials
- The sequence of the target gene and the Gene Bank ID
- Plasmids, Agrobacterium, etc.
- Vector to be transformed (conventional vector can also be provided by us)
※ We do not accept the transformation service of lethal genes or severely defective genes. If there are any of the above, please inform our company in advance.
Arabidopsis species that can be transformed
- Genetic transformation of Col-0 / ler (wild type)
- Genetic transformation of mutants/other varieties
Workflow of Arabidopsis transformation
- Vector construction and transformation of recombinant plasmid into Agrobacterium (optional)
- Agrobacterium infection of Arabidopsis thaliana inflorescence operation (3 times)
- Screening of T0 generation transgenic positive plants
- Harvesting of T1 generation seeds
Feedback to customers
- T1 generation seeds (T1 generation positive lines ≥12)
- PCR identification of inserted genes in T1 generation seeds
- T2 or T3 generation seed (optional)
Service process
Online order — Order/experimental material confirmation — Culture required Arabidopsis seedlings — Agrobacterium infection inflorescence — Harvest T0 generation seeds — Screen for transgenic positive seedlings — Collect T1 generation seeds (optional) — Express genetically modified seeds or seedling according to customer requirements
Project cycle
Takes about 3~4 months
Advantages
※ It should be noted that our service is only used for research.
Online Inquiry
