Services
- Genetic Marker Assisted Breeding
- Plant Molecular Biology Research
- Research on DNA Level of Plant
- Research on Microspore Embryogenesis
- Plant Genetic Engineering
- Plant Genome Editing with CRISPR / Cas9
- Plant Genome Editing with TALEN
- Plant Genome Editing with ZFN
- RNAi Mediated Plant Gene Silencing
- Overexpression of Plant Genes
- Arabidopsis thaliana Transformation
- Oryza sativa Transformation
- Zea mays Transformation
- Triticum aestivum Transformation
- Medicago truncatula Transformation
- Glycine max Transformation
- Gossypium hirsutum Transformation
- Nicotiana tabacum Transformation
- Solanum lycopersicum Transformation
- Brassica napus Transformation
- Solanum tuberosum Transformation
- Virus-Induced Gene Silencing (VIGS) of Plant Genes
- Research on Protein Level of Plant
- Plant Epigenetic Modification Testing Services
- Sequencing-based Plant Breeding
- Plant DNA-level Sequencing Services
- Plant Genome De Novo Service
- Plant Whole Genome Resequencing Service
- Plant Reduced-Representation Genome Sequencing (RRGS)
- Plant Genetic Map Service
- BSA Trait Positioning of Plant
- Genome-Wide Association Study (GWAS) of Plant
- eQTL Analysis of Plant
- Plant Genetic Evolution Service
- Plant Pan-genome Sequencing
- Plant Whole Exome Sequencing Service
- Individual Selection Pressure Analysis of Plant
- Mixing-tank Selection Pressure Analysis of Plant
- Plant Whole Genome Survey
- Plant RNA Level Sequencing Services
- Eukaryotic Transcriptome Sequencing without Reference Genome
- Eukaryotic Transcriptome Sequencing with Reference Genome
- Prokaryotic Transcriptome Sequencing Analysis
- LncRNA Sequencing of Plant
- Plant Small RNA Sequencing
- Plant Circular RNA Sequencing
- Plant Comparative Transcriptome Service
- Plant Isoform-sequencing with Reference Genome
- Plant Isoform-sequencing without Reference Genome
- Ribo-seq of Plant
- Metatranscriptome Sequencing of Plant
- Plant Single Cell Level Sequencing Services
- Plant Epigenetics Level Sequencing Services
- Plant Proteomics Service
- Proteomics Qualitative Analysis in Plant
- Plant Protein Quantitative Analysis Service Based on Isotope Labeling (iTRAQ / TMT)
- Non-labeled Plant Protein Quantitative Analysis (Label-free / DIA)
- Plant Protein Targeted Quantitative Service (PRM / MRM / AQUA)
- Post-translational Modification Proteomics (PTMs) Service for Plant
- Plant Metabolomics Services
- Plant DNA-level Sequencing Services
- Other Services
- Plant CNV Analysis Service
- Plant Mutation Detection Service
- Plant Strain / Cell Level Services
- Plant Tissue and Cell Culture Services
- Plant Polyploidization Services
- Plant Haploidization Services
- Plant Phenotypic Analysis
- Plant Stress Response Indicators Analysis
- Plant Biochemical Analysis
- Plant Tissue and Cell Imaging Services
- Plant Disease Identification Services
- Plant Organelle Isolation Services
- Genetically Modified Plant Testing Services
- Seed Testing Services
Plant Organelle Isolation Services
INQUIRYIntroductions
Organelles are functional organs in cells that maintain the complex life activities of cells. In order to study the structure and function of various organelles individually and clearly, these organelles must first be separated from the cell. Various physical methods (grinding, ultrasonic vibration, hypotonic, etc.) are used to homogenize the tissue, and various subcomponents in the cells are released from the cells. With the continuous improvement of plant cell separation technology, more and more researches are focused on the level of organelles, providing powerful research methods and important resources for plant breeding.
Services
With years of experience, Lifeasible provides a one-stop service for separating different types of plant organelles, including key steps from sample homogenization and purification to organelle enrichment and integrity testing.
Service content
- Separable main organelles
※ The items listed are constantly being updated, please contact our staff via email for more updated information.
- Cell disruption and homogenization
- Plant organelle purification methods
The cell wall is removed by enzyme digestion, and then the cells are homogenized by Dounce homogenization, filtration, grinding, sonication and solubilization.
Differential centrifugation
Density-equilibrium centrifugation
Affinity chromatography
Free-flow electrophoresis (FFE)
Affinity purification
Fluorescence-activated organelle sorting (FAOS)
Microfluidic technology
※ The listed methods are free for you to choose. If you don't understand, please contact our staff to give you the best solution.
- Purity, enrichment, and integrity detection of organelles
We use the most commonly used methods in scientific research to analyze the integrity and purity of plant organelles---microscopic technology and labeled enzyme activity analysis technology.
Delivery standard
Complete experimental report, including methods, original experimental records, instrument parameters, reagents, consumables and experimental conditions, etc.
Service process
Advantages
- We have established an excellent plant organelle separation platform and mastered the most advanced separation technology.
- Leading technology platform conditions, first-class R&D and production equipment.
- Our experienced scientists and experts will wholeheartedly provide you with assistance in project design, experiment optimization, data interpretation, technical consultation, etc.
- The one-stop service we provide makes your upstream and downstream experiments seamlessly connected, facilitates your experiments more conveniently and quickly.
- Standardization system, scientific management.
※ It should be noted that our service is only used for research.
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