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Cold Stress Tolerance Breeding
INQUIRYIntroductions
Cold resistance of plants refers to a cold resistance that plants acquire through their genetic variation and natural selection during long-term adaptation to low-temperature environments. Breeding for cold resistance is the fundamental way to improve the cold resistance of plants and mitigate cold damage. At present, the ways of cold resistance breeding generally include the selection of bud sport, actual breeding, mutagenesis breeding, biotechnology breeding, and cultivation of new cold-resistant varieties through rootstock varieties with strong cold resistance. Breeding ornamental flowers with cold resistance can expand the survival area and growth time of plants and has been the focus of research by breeders.
Lifeasible is dedicated to genetic engineering research for cold resistance of ornamental flowers and expects to breed cold-tolerant ornamental flower varieties through genetic engineering technology.
How We Help
Genetic engineering improves cold resistance in ornamental plants mainly by introducing cold resistance regulatory genes and cold resistance functional genes. During low-temperature stress, ornamental plants develop a set of pathways to sense and conduct signals and produce a series of changes to enhance their resistance to low-temperature cold damage. The low-temperature response pathway consists of multiple components, including extracellular signals, the transmembrane transmission of signals, intracellular second messengers, transcription factors, functional genes, effectors, and other components.
Figure 1. The mechanism of cold tolerance in plants. (Ritonga FN, et al., 2020)
What We Provide
Cell Engineering Assisted Cold Stress Tolerance Breeding
We can use cell engineering technology to help our customers grow ornamental flowers with cold tolerance. Based on single-cell culture, we use different low-temperature treatments to screen for cold-resistant cell lines and then induce them to differentiate into frost-resistant and cold-tolerant plants. Based on tissue culture or protoplast culture, we can introduce exogenous cold-resistant genes and then screen the transformed cold-resistant cell lines by low temperature. Also, we can select cold-resistant mutants by protoplast fusion cell hybridization.
Gene Engineering Assisted Cold Stress Tolerance Breeding
Genetic engineering to modify the cold resistance properties of ornamental flowers is divided into four main steps. First, we study the status of altered gene expression in plants comparing low and non-low temperature conditions, followed by isolation of genes associated with low-temperature stress, then transfer of cold resistance-related genes, and finally, genetic transformation or transfer using the isolated genes. In addition to genes of plant origin, we also considered obtaining cold resistance-related genes for transfer into ornamental flowers from a broader range of species, working to break the limitation of the lack of frost-resistant germplasm resources in the plant genetic population.
Lifeasible provides the most comprehensive and efficient solutions for breeding ornamental flowers. We are committed to helping our customers cultivate more ornamental flowers. We look forward to providing you with the most professional and efficient service to meet your individual needs. If you are interested in our services or have further questions, please do not hesitate to contact us.
Reference
- Ritonga FN, Chen S. Physiological and Molecular Mechanism Involved in Cold Stress Tolerance in Plants. Plants (Basel). 2020 Apr 28; 9(5): 560.
※ It should be noted that our service is only used for research.
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